FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295599
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC795766.142023882389536Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG38340.2959248965150482No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA27780.21441819575347001No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT23350.18022551730898217No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA19680.1518988514193049No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT14400.11114550103851577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)