FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408576
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTA16976212.052029851424418TruSeq Adapter, Index 23 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG74390.5281220182652552No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA53790.3818750283974737No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT45190.3208204598118951No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATATCGTA40060.2844006997137534TruSeq Adapter, Index 23 (97% over 37bp)
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA37080.2632445817620065No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT28050.19913728474714887No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT19280.13687582352673905No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT18470.13112533509019036No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAACCTCGTA14290.10144997501022308TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)