FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905979
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTA21292611.171476705671992TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATATCGTA112100.5881491873729984TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCCTAATCTCGTA22430.11768230394983364TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT19300.1012602972015956No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATTTCGTA19260.10105043130065965TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)