FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491396
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGTA638214.2792792792792795TruSeq Adapter, Index 23 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT43890.29428803617550264No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG31080.20839535576064305No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA28190.189017537930905No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT24650.16528138737129508No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA17080.11452357388647952No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA16780.11251203570346172No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT16150.10828780551912437No Hit
TGTATCGCCAATCACACCGGTTGAGACCGCACCAGCGACACAAGATCGGA15260.10232024224283826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)