FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773192
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTA1034365.833322054238909TruSeq Adapter, Index 25 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT32140.18125504739475476No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG23910.13484157383971956No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATATCGTA17850.1006659177347969TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph