FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705263
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTTCAATCTCGTA834534.893849218566285TruSeq Adapter, Index 10 (97% over 39bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG79040.4635062157567484No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT57000.33425929020919354No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA53870.3159043502380571No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTTCAATATCGTA46250.2712191609153544TruSeq Adapter, Index 10 (97% over 39bp)
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT38510.22583026782379023No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA32560.19093828928440948No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT26890.15768828620570552No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT19550.11464507234367953No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA17590.10315124411894236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)