FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996470
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC146131.4664766626190453Illumina Single End PCR Primer 1 (98% over 50bp)
NTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC32920.33036619266009015No Hit
NCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG17820.17883127439862714No Hit
NTGTTTTCAGCCTGGTTCATTTGGTACAAGTGACTCAGAAAATGGCCTTT14960.15012995875440305No Hit
NTGCTGTCACCTATTTCTTTGACCTCCACCAACCCTGGGAAATTGCTGCT13600.13648178068582095No Hit
NTCCAGCCTCAAACAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGG12330.12373679087177739No Hit
NTCCAAGGACCGCAGACTTCTGCTGAAGTGTACCGCATCCTCAAACAGAA12150.12193041436270033No Hit
NTGCCCTCCTGTTTCGATTACGTGATGCACTTTCTGACTGTGTTCTGGAA12100.12142864311017892No Hit
NCATGCTCACACACAAGCACACATGATCACACAGGCACACATGATCACAC11810.1185183698455548No Hit
NTTCAGCGTCTCAACCACCATGGGGCTCATCTCACCTGCCATGGTGAAGA11720.11761518159101629No Hit
NTCCCAACTTACACCGGCTCCATCTACCCCACCGTGAAGCCCTACCACTA11550.11590915933244353No Hit
NTGGGAGGTGCAGAACAGGACAGACCAGCGGGGTGGCATAGAGAAAGTCC11430.11470490832639216No Hit
NCATGCTCACACAATTACATACACCGACTCACGCACACATGCTCACACAA11330.11370136582134936No Hit
NTCCTCACACGCCATGAACTGGGAAACCTGCAATACACACAGAGCATGGG11240.11279817756681085No Hit
NGGTGGCCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGT11240.11279817756681085No Hit
NCATGCTCACCCACACACACGCTCACACATACACTCACACACACCTGCTC10940.10978755005168245No Hit
NTCCATAGGGAAGCCTGTGTCTCCTGCCCCCAGGGCGCACCCTCAGTGCA10900.10938613304966532No Hit
NTCTCCGGATACTCGAAGATAAGCCACACCTGGCGCTGGAACTCGTTGCG10870.10908507029815248No Hit
NTGATCTCCAAGGTCCTTCCTAAGAATCTGGGATCTGGAGGATCCCAGGG10360.10396700352243421No Hit
NTGTGTCCACAAATGCACACCTACAAACAGACCCAGGAGTACAACTCCAC10110.1014581472598272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)