FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001809405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357649
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACGCAAATCTCGTA716595.27816836310416TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACGCAAATATCGTA34890.25698836739098246TruSeq Adapter, Index 18 (97% over 40bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT32810.24166776537971152No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG27850.2051340221220654No Hit
CTCAGTACCTAACTGCCAATCAACACACTGCCAATGCGGGTGGGCTAAGC18500.13626496981178493No Hit
TCATGGTCTCTGCTTACTACTTGATTCTCTCCTGCATGTTTTTGATGCCT18030.13280310301116122No Hit
CTGGGGGACGAGGCCATGGAGCGCTTCCGCGAGGATGAGGGCTTCATTAA17960.13228750582808957No Hit
CCGTGGGGCTGGAAGTCCTGAGAATAGGAATGATGGACATTTATCTGGTC17640.1299304901340479No Hit
ACATGATAAATGGGCCTGTGACAAGGTAGGGATTATCATCCAACTGACAA17630.1298568333936091No Hit
CTCGTTCTCATAGCAGAAGGAGTTGGCACTGGGCAGCAGGAACTTGTTCT17630.1298568333936091No Hit
CTCGTTGCCTACTGGGAGAAGACTTTCAAGATTGACCTATTCAGGCCACA17200.1266895935547406No Hit
CTCGTTCTCGTAGCAGAAGGAGTTGGCGCTGGGCAGCAGGAACTTGTTCT17140.12624765311210775No Hit
CTCGTTCTTGTAGCAGAAGGAGTTGGCCCTGGGCAGCAGGAACTTGTTCT16780.12359601045631087No Hit
GGATGGAGACAATGAAACACGCCCAGCCATTCCAGTATTCAGTAGGGGGG16770.12352235371587206No Hit
GTATGGGTGAGCATGTATGGGTATGTGTGAGCAGGTGTGTGTGAGTGTAT16580.12212287564753482No Hit
CTTATGGAGCCCCTCCCTGGGAGTGGGGAGCCCACAGAGGCAGTTCCCGT16550.12190190542621841No Hit
CTGGGTCCAACAAAAGCACTGCCAGTAGCAAATCAGGGGATGGGAAGACC16270.11983951669393195No Hit
CTCCTCGGGGAATGACAACACGGAGAACGGCTTGCCTGGCTCCAAGGCAG16130.1188083223277887No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA15890.11704056055725744No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA15690.11556742574848139No Hit
CTCGTTCTCGTAGCAGAAGGAGTTGCGCTGGGCAGCAGGCACTTGTTCTC15590.11483085834409336No Hit
CTCATGTATTTCAGAGCCCAGGCTACAGAGACTGCAGCAAGGTGGCTGGG15420.11357869375663371No Hit
GTATGACAATGGACAGTGCTGCCTGAGGGCTGCAGGTGCATGGCTGCGAA15370.11321041005443969No Hit
CTCCTTGGAGATGTCCCCTTTGGCCCCAGTAAACAGCCATGAGAGAAGAA15340.11298943983312328No Hit
CTTTGGCTAGAGGAGTAAAAATCGCATAACTTTGAAAAGTAGACAAATTC15270.11247384265005167No Hit
GTGTGAACCTCTTCGCAGGGAAGTTTTGGAGGTGCATCAACTATACCGAT15190.11188458872654125No Hit
AGATGACCAAGACGAAGAAGATCATGTATGTTTTCCCGGCTGCTCGTAAA15160.11166361850522483No Hit
TGGTGTGTTCGTTCATGTCAACCATACACCCCACGACATCGCCTGCTTGC14990.11041145391776519No Hit
GTGTGAGCATGTGTAATCCTGTGTGTGCCTATACATGTGAGAGTGTGTGC14840.10930660281118317No Hit
CAGTGGCAAAGATGATGATGGCCATGGTGAGGGAGAAGAGAAGAAAGCCC14740.10857003540679513No Hit
CTACGGGGCCAGCGTGGGGGGACTCACATCCACGATGAGGAAGTCGAGCC14680.10812809496416231No Hit
CTATGGGTTACCTTCTCTTTGGGACCGATGGGTGCTGGGGAGGATCCCCC14530.10702324385758027No Hit
TGGTGGTGTCCTTGGTGTCCCTGGCCTTGAATATCATTGAACTCTTCTAC14400.10606570623187585No Hit
CTTTGTACGTTCCCTTTCACGAAAAAGAACCACCGTGAGCAACGTCCGCG14220.1047398849039774No Hit
CTGGACGCCGACGATGCCGAAGATGAAGAAGACGAAGAAGCAGAGCAGCA14220.1047398849039774No Hit
CTACTCGTCCACCGGCTCCAACGCCAACATCAACAACGCCAACAACACCG14050.10348772031651773No Hit
CTCGTCTCCCTCAGACACTACTACTTCACTATAGGCTGGAACATCTTTGA13950.10275115291212972No Hit
CTCACAGCCTGGCCGCCCTCCCACCACTGCCCTTCCTGCTGTTACAGTTG13890.1023092124694969No Hit
TGGTGGTGTCCTTGGTGTCCCTGGCCTTGAATATCATTGAACTCTTCTAT13790.10157264506510887No Hit
CTAGATGTGTCTAGAAGCCCCCCTCTCATGGTCAAAAAGAACCCAGCCTT13780.10149898832467008No Hit
CTTTACCGACGACGCTTTCCCCGGAAGACCCTGGGGCCCTCTGATCTCTC13750.10127801810335367No Hit
GTGTGTAAGTAGAGAAGAGGGTGGTTAGGCCAGGCTCTGATGGCAAGGGG13750.10127801810335367No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)