FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357649
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC718705.293709935336747Illumina Single End PCR Primer 1 (100% over 50bp)
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG62170.45792395530803615No Hit
CTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC48840.3597395203031122No Hit
CTCCTCACACGCCATGAACTGGGAAACCTGCAATACACACAGAGCATGGG40140.2956581561213539No Hit
CTCCATAGGGAAGCCTGTGTCTCCTGCCCCCAGGGCGCACCCTCAGTGCA35530.26170239877906587No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT30470.2244320881170317No Hit
CTCGTGCTCTGGGGCCAAAGCCCAGTAAACCAAGTTAACAAACCAAGCCA26340.19401185431580623No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG25520.1879720015998244No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGGGTGTAGATCTCGGTGGTCGCC24790.18259505954779182Illumina Single End PCR Primer 1 (98% over 50bp)
CTCCAGCCTCAAACAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGG23200.17088363781802218No Hit
CTGGCAAGACCTACATGATCTTCTTCGTGGTCATCATCTTCCTGGGCTCT21050.15504743862367962No Hit
TGGTGGCCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGT20220.14893392916725898No Hit
CTCTCCGGATACTCGAAGATAAGCCACACCTGGCGCTGGAACTCGTTGCG17160.12639496659298538No Hit
CTGGGAGGTGCAGAACAGGACAGACCAGCGGGGTGGCATAGAGAAAGTCC17070.12573205592903616No Hit
CTGCTGTCACCTATTTCTTTGACCTCCACCAACCCTGGGAAATTGCTGCT16950.1248481750437705No Hit
CTGTGACATTCACCACCCCAATCTGTGGCCTGAATAGGTCAATCTTGAAA16950.1248481750437705No Hit
CTGGCTGGCTCAAGGATGGGCAGCGTCCATCTGCTGCAGGCATCCTGGCG16910.12455354808201531No Hit
CTCCAAGGACCGCAGACTTCTGCTGAAGTGTACCGCATCCTCAAACAGAA16850.12411160763938249No Hit
CTCTAATCAGCGCGGGGATTTATCTCCCGGCCTGTCAGCATGTGTGCTTA16350.12042877061744235No Hit
CTCTGGGAGGCCCGGCGCAGCCGCCTCGGGCCCAGCGTAGGCCTCTGGCT16010.11792444144252308No Hit
CTTAGGCCCGGTTCATCTCAGATGTGTTTTCTAGGCCTGGGGCTGAAAGG15860.11681959033594103No Hit
CTGCTCAGCCCGAAGCAGGGCTCACACTTTTGAAGGTGGTTTCTCCTCTG15850.11674593359550223No Hit
CTCCAACAACTGTCCTCCCCAGCCTCACCCCTCTATTCCACCCTCCCCAG15380.1132840667948785No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA15350.11306309657356209No Hit
CTGCCCTCCTGTTTCGATTACGTGATGCACTTTCTGACTGTGTTCTGGAA15320.11284212635224568No Hit
CTCTGAGCATCTTCCCCACCATCATCCTCCAAGTCATCAAGATCAGATTC15200.11195824546698005No Hit
CTGCCAGTGGAGTTTTGAATCTTGCAGTCAGACTTGTTATTCACAATCGA15150.11158996176478604No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA15040.11077973761995921No Hit
CTGCTGCTTACCATAAACTATGAAAAGCACAGGTTTTAAGCCCGCCAGGA14980.11033779717732639No Hit
CTGTGCCTCCGAACTGGGCTTTCTTCTCTTCTCCCTCACCATGGCCATCA14960.11019048369644878No Hit
CTGGGATTACCATTGCTCTGGGACCATCTTCTGAGTCAGACTTGGGGAGC14900.10974854325381596No Hit
CTTGCCATGTACAAGGGGGACCTGTTTCAGATATTCCATGGAGACCCTGG14840.10930660281118317No Hit
CTCCTATCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC14790.10893831910898916No Hit
CTGGGGGCAGCATTTCTGGAATCCTCCTAACCATTGTTCACTGCTTGTAG14610.10761249778109071No Hit
CTGTTTGGCGGCAAGTTTAATTTTGATGAAACGCAAACCAAGCGGAGCAC14480.10665496015538627No Hit
AGATGCTGCTCAAATGGGTGGCCTACGGCTTTAAGGTGTACTTCACCAAC14450.10643398993406986No Hit
CTGTTTTCAGCCTGGTTCATTTGGTACAAGTGACTCAGAAAATGGCCTTT14430.10628667645319226No Hit
CTGATCTCCAAGGTCCTTCCTAAGAATCTGGGATCTGGAGGATCCCAGGG13800.10164630180554768No Hit
CTTTGCTACATAAACACATAAACCTGTTTCTCAACCCAGGGGTAAGACTT13630.10039413721808803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)