FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756874
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAACGAATCTCGTA881265.016068312240947TruSeq Adapter, Index 6 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAACGAATATCGTA69500.39558898361521655TruSeq Adapter, Index 6 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT48850.27805067409501194No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG35520.20217727623039558No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA28420.16176458869560367No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA21760.1238563494024045No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA17870.10171475017559596No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)