FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230695
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCGGTAATCTCGTA681565.538009011168486TruSeq Adapter, Index 9 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT20700.16819764442042912No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG20140.16364736998200202No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCGGTAATATCGTA16130.13106415480683678TruSeq Adapter, Index 9 (97% over 37bp)
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA16120.13098289990615058No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCGGCAATCTCGTA14220.11554446877577304TruSeq Adapter, Index 9 (97% over 37bp)
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA13510.109775370827053No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)