FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340246
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTA1217909.08713773441592TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATATCGTA48900.36485839166839523TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT19560.14594335666735808No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG14330.10692066978748678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)