FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733648
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCTCGTA24299914.016628519745646TruSeq Adapter, Index 10 (97% over 36bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT85370.49242983581442135No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG80260.4629544175057451No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATATCGTA48720.28102590606628336TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCGGTAATCTCGTA44670.2576647623969803TruSeq Adapter, Index 4 (97% over 37bp)
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA41430.23897584746153774No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA41090.23701466503004068No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA36640.21134624791191753No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT36460.21030797485994848No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT35370.20402065471191383No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGCAATCTCGTA33970.19594519764104362TruSeq Adapter, Index 10 (97% over 36bp)
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA26970.1555679122866926No Hit
CTGTGTAGCAGAAGACAGGGCATGAGACCGCACCAGCGACACAGAGATCG25840.1490498647937759No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA23320.1345140420662095No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT23170.133648814522902No Hit
TGTATCGCCAATCACACCGGTTGAGACCGCACCAGCGACACAAGATCGGA22530.1299571770047899No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT22140.12770758539219035No Hit
TGTGGAGATGGTCGCATTGCTGAGACCGCACCAGCGACACAAGATCGGAA17910.1033081686709182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)