FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644634
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATCTCGTA26397116.05044040193745TruSeq Adapter, Index 1 (97% over 36bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG101990.6201379759873625No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA73600.44751598227934No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT59300.3605665455049573No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA39950.2429111887508102No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT38010.23111525117442544No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATATCGTA31870.19378171678318704TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCACCTCGTA28910.1757837914089092TruSeq Adapter, Index 1 (97% over 36bp)
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT25330.1540160303143435No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT24790.1507326250095766No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTCACCATTTCGTA17050.10367048230791776TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)