FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644634
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC26604316.176425879557396Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG94390.5739270865128655No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA68790.4182693535461386No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT56760.34512237981216487No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA38490.23403383366755157No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT34950.21250928778074638No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT23890.14526028283496512No Hit
TGTGTCGCTGGTGCGGTCTCAAATATATTTTGCCTCAATCAACACAAGAT23100.14045678248169502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)