FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465137
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATCCTCATCTCGTA16952311.57045382104199TruSeq Adapter, Index 16 (97% over 39bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG82580.5636332984560488No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA58390.3985292842921856No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT46020.3141003196288129No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA37010.25260436396050334No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT31980.21827310347086998No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT19030.1298854646357303No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATCCTCATATCGTA17950.12251414031588856TruSeq Adapter, Index 16 (97% over 39bp)
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT17060.11643962305231526No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATCCTCACCTCGTA16940.11562058701677727TruSeq Adapter, Index 16 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)