FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572949
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTA802475.101691154640106TruSeq Adapter, Index 1 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT35690.22689864706357293No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG25750.16370524409882328No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA22350.1420897943925709No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA16250.103309134625471No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)