FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001809461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6964703
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTA3405824.890115199456459TruSeq Adapter, Index 16 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT292700.42026199825032023No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG214090.30739286370143853No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA176640.25362172658331594No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATATCGTA169270.24303979652829416TruSeq Adapter, Index 16 (97% over 37bp)
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA124170.17828470216174327No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA113780.1633666216635512No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT110280.15834128174596965No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA102230.14678299993553207No Hit
TGTATCGCCAATCACACCGGTTGAGACCGCACCAGCGACACAAGATCGGA98640.1416284369914984No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT94930.13630157667886197No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA93020.13355917689526747No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT89100.12793079618757613No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT83560.11997640100374703No Hit
TGTGGCAAACCACTGGTGTCTGAGACCGCACCAGCGACACAAGATCGGAA78810.11315629682988636No Hit
CTGTGTAGCAGAAGACAGGGCATGAGACCGCACCAGCGACACAGAGATCG72490.1040819687501391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)