FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6964703
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3577405.136471720330357Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT284320.408229898676225No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG206420.2963801902249098No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA172260.24733287262931383No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA119790.17199584820774125No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA111770.16048064073945434No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT109450.1571495582798003No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA99440.14277708611551704No Hit
TGTGTCGCTGGTGCGGTCTCAACCGGTGTGATTGGCGATACAAGATCGGA96010.13785225299628714No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT92320.13255410891175115No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA90970.1306157635149697No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT85570.12286238192784388No Hit
TGTGTCGCTGGTGCGGTCTCATTTGTTTTTGTTTCCACTTGACACAAGAT81560.11710477819370044No Hit
TGTGTCGCTGGTGCGGTCTCAGACACCAGTGGTTTGCCACAAGATCGGAA74690.10724075384119036No Hit
CTGTGTCGCTGGTGCGGTCTCATGCCCTGTCTTCTGCTACACAGAGATCG70100.1006503794921334No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)