FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101813
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTTACATCTCGTA100560.9126775596221863TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG23270.21119736289188817No Hit
CTGTGTAGCAGAAGACAGGGCATGAGACCGCACCAGCGACACAGAGATCG17110.155289509199837No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA13420.12179925268625438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)