FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635604
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTA17227310.53268395039386TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATATCGTA102570.6271077840357446TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACAACAACCAATCTCGTA67080.41012372187889Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTA48090.2940198238693474Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG37310.2281114499597702No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATTTCGTA27200.16629942210950818TruSeq Adapter, Index 1 (97% over 37bp)
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA25580.1563948241750448No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT19370.11842719875960195No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA18770.11475882915424516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)