FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818967
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTA136711.6692980303235663TruSeq Adapter, Index 13 (97% over 38bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTA15330.1871870295140097TruSeq Adapter, Index 13 (97% over 38bp)
ACATGATAAATGGGCCTGTGACAAGGTAGGGATTATCATCCAACTGACAA10400.12698924376684287No Hit
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTA9800.1196629412418327TruSeq Adapter, Index 13 (97% over 35bp)
AGATGACCAAGACGAAGAAGATCATGTATGTTTTCCCGGCTGCTCGTAAA8320.10159139501347428No Hit
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACAGCCATGCATCTCGTA8210.10024823955055576TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)