FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551693
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAACGAATCTCGTA856325.518617406922632TruSeq Adapter, Index 6 (97% over 38bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG77520.49958335830605666No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT58550.3773297939734213No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA57080.36785627053805103No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAACGAATATCGTA45540.29348588928351166TruSeq Adapter, Index 6 (97% over 38bp)
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT43670.2814345363419182No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA35390.22807346556309788No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT31000.19978178673229818No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT21530.1387516731724639No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA20420.13159819629269448No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA16480.10620657565639595No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT15910.10253316861002788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)