FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551693
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC865115.575265210321887Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG68150.4391977021227782No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA50920.3281576961422137No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT50610.3261598782748907No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT40690.2622297065205553No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA32200.2075152752509678No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT27730.17870803051892353No Hit
TGTGTCGCTGGTGCGGTCTCAAATATATTTTGCCTCAATCAACACAAGAT19110.1231558046598135No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA18930.12199578138201307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)