FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422445
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACAGAAATCTCGTA938456.5974431348839495TruSeq Adapter, Index 23 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG89230.6273001768082421No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT75330.5295811085841632No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA64080.4504919346617971No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT45570.3203638805015308No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA41010.28830640200499846No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT33790.23754872771882216No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA23180.1629588490240396No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT21590.15178091244301184No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT19990.14053267437405315No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACAGAAATATCGTA19900.1398999609826742TruSeq Adapter, Index 23 (97% over 37bp)
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA18980.13343222409302294No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT17550.12337911131889107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)