FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544705
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTA1337938.661394894170732TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATATCGTA78690.5094176557983564TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACACTATGCAATCTCGTA49300.3191547900731855TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)