FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513959
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGGTCATCTCGTA901485.954454512968978TruSeq Adapter, Index 2 (97% over 36bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG96030.6342972299778263No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA66720.44069885644195117No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT60770.4013979242502604No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT48180.3182384727723802No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA41580.274644161433698No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT29240.1931360096277376No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT22920.15139115392160554No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA19340.12774454261971427No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT16790.11090128596613251No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGGCCATCTCGTA16370.10812710251730728TruSeq Adapter, Index 2 (97% over 36bp)
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA16250.10733447867478578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)