FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812121
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTA1412747.7960577687693045TruSeq Adapter, Index 10 (97% over 36bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG72950.40256693675532707No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT67890.3746438565636621No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATATCGTA66420.36653181548031283TruSeq Adapter, Index 10 (97% over 36bp)
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA36350.20059366896581407No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT33450.18459032261090733No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA30340.16742811324409354No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT29880.16488965140848763No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA25610.14132610349971111No Hit
CTGTGTAGCAGAAGACAGGGCATGAGACCGCACCAGCGACACAGAGATCG21010.1159414851436521No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA19520.10771907615440691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)