FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812121
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1490978.22776183268115Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG69000.38076927534088506No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT64910.35819903858517177No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA35130.19386122670616365No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT33230.18337627564605233No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA29680.16378597234952855No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT28680.1582675770547331No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA24990.13790469841693792No Hit
CTGTGTCGCTGGTGCGGTCTCATGCCCTGTCTTCTGCTACACAGAGATCG19720.108822755213366No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA19090.10534616617764486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)