FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738303
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA108881.4747332734663139TruSeq Adapter, Index 10 (97% over 38bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA35740.48408309325575TruSeq Adapter, Index 10 (97% over 38bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA11700.1584715218548482TruSeq Adapter, Index 9 (97% over 35bp)
ACATGATAAATGGGCCTGTGACAAGGTAGGGATTATCATCCAACTGACAA9730.13178871005535667No Hit
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA8920.12081760469617488TruSeq Adapter, Index 9 (97% over 35bp)
AGATGACCAAGACGAAGAAGATCATGTATGTTTTCCCGGCTGCTCGTAAA8370.11336808871154527No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)