FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738303
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC145851.9754761933785991Illumina Single End PCR Primer 1 (98% over 50bp)
NTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC14770.20005336562359896No Hit
NTGCTGTCACCTATTTCTTTGACCTCCACCAACCCTGGGAAATTGCTGCT7760.10510589825586514No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)