FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369380
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1041887.608406724210957Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT113690.8302297390059734No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG103790.7579342476157093No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA59240.4326045363595204No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA53550.39105288524733817No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT48550.35454001080781083No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT45360.3312447969153924No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA44430.32445340226964026No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA37520.27399260979421347No Hit
TGTGTCGCTGGTGCGGTCTCAACCGGTGTGATTGGCGATACAAGATCGGA33960.24799544319326997No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA33600.245366516233624No Hit
CTGTGTCGCTGGTGCGGTCTCATGCCCTGTCTTCTGCTACACAGAGATCG31260.22827849099592515No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT30840.2252114095430049No Hit
TGTGTCGCTGGTGCGGTCTCATTTGTTTTTGTTTCCACTTGACACAAGAT28470.20790430705866889No Hit
GGCACGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGATC26440.1930800800362208No Hit
TGTGTCGCTGGTGCGGTCTCAGACACCAGTGGTTTGCCACAAGATCGGAA24130.17621113204515912No Hit
TGTGTCGCTGGTGCGGTCTCAGCAATGCGACCATCTCCACAAGATCGGAA22940.1675210679285516No Hit
TGTGTCGCTGGTGCGGTCTCAAATATATTTTGCCTCAATCAACACAAGAT18150.13254173421548437No Hit
TGTGTCGCTGGTGCGGTCTCATGGACATGGGCATGGACACAAGATCGGAA15850.11574581197330179No Hit
TGTGTCGCTGGTGCGGTCTCAGTCTCTTGGACATAAACTTGACACAAGAT14040.10252815142619287No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)