FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516196
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA747554.930431157976937TruSeq Adapter, Index 7 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATATCGTA107630.7098686449509167TruSeq Adapter, Index 7 (97% over 39bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA25360.16726069716580177TruSeq Adapter, Index 7 (97% over 39bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT25270.16666710636355722No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG19080.12584125007584773No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA16780.11067170735181994No Hit
TGTGTCGCTGGTGCGGTCTCATTGGAGGGGTCTCTGCCACAAGATCGGAA16420.10829734414284169No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)