FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518884
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1025346.750614266790618Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG73020.48074770686899065No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT64980.42781410561965233No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA51960.34209327374572385No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT35860.2360943956220488No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA33550.22088586093473891No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT28610.18836198156014547No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA19930.1312147603108598No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT17910.11791552218602605No Hit
TGTGTCGCTGGTGCGGTCTCAAATATATTTTGCCTCAATCAACACAAGAT17000.11192428124860096No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA16060.1057355268736783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)