FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160906
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTAGAATCTCGTA436133.7568071833550696TruSeq Adapter, Index 20 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG27670.2383483244982798No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT14620.12593612230447598No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA14390.12395491107807179No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT13390.11534094922414045No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)