FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441426
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTA1003936.964838985837636TruSeq Adapter, Index 2 (97% over 36bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG107010.7423898278510308No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT99090.687444239246413No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA75770.5256600061328157No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT56950.3950948574536605No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA46090.31975280035187376No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT44750.31045645076472883No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA29980.2079884780765714No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT26400.18315196201539308No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA24930.1729537277668087No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT24830.1722599703349322No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATATCGTA23810.16518364452979203TruSeq Adapter, Index 2 (97% over 36bp)
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT23180.16081297270897013No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA16790.1164818728120625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)