FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408833
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTCACAATCTCGTA1139518.08832558578625TruSeq Adapter, Index 8 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTCACAATATCGTA40390.28669118341208644TruSeq Adapter, Index 8 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG28450.2019401873749408No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT21520.1527505389212206No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)