FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915192
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC256282.80028671579297Illumina Single End PCR Primer 1 (100% over 50bp)
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG41160.44974169354627225No Hit
CTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC34010.37161601062946353No Hit
CTCCTCACACGCCATGAACTGGGAAACCTGCAATACACACAGAGCATGGG27120.2963312616369024No Hit
CTCCATAGGGAAGCCTGTGTCTCCTGCCCCCAGGGCGCACCCTCAGTGCA22710.24814465161408752No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT21630.2363438491595206No Hit
CTCGTGCTCTGGGGCCAAAGCCCAGTAAACCAAGTTAACAAACCAAGCCA17530.191544506507924No Hit
CTCCAGCCTCAAACAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGG16040.17526376978819744No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG15470.16903556849273157No Hit
TGGTGGCCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGT14570.15920156644725916No Hit
CTGGCAAGACCTACATGATCTTCTTCGTGGTCATCATCTTCCTGGGCTCT14310.15636063252301155No Hit
CTGGCTGGCTCAAGGATGGGCAGCGTCCATCTGCTGCAGGCATCCTGGCG12570.13734822856843154No Hit
CTCCAACAACTGTCCTCCCCAGCCTCACCCCTCTATTCCACCCTCCCCAG12370.1351628947805488No Hit
CTCTGGGAGGCCCGGCGCAGCCGCCTCGGGCCCAGCGTAGGCCTCTGGCT12180.13308682768206015No Hit
CTCTCCGGATACTCGAAGATAAGCCACACCTGGCGCTGGAACTCGTTGCG11940.13046442713660084No Hit
CTGTGACATTCACCACCCCAATCTGTGGCCTGAATAGGTCAATCTTGAAA11700.12784202659114152No Hit
CTCCTATCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC11550.12620302625022944No Hit
CTGCTGTCACCTATTTCTTTGACCTCCACCAACCCTGGGAAATTGCTGCT11230.12270649218961704No Hit
CTTTGCTACATAAACACATAAACCTGTTTCTCAACCCAGGGGTAAGACTT11230.12270649218961704No Hit
GCATGCGCATCCTTGTCACGTTGCTGCTGGATACGCTGCCCATGCTGGGC11160.12194162536385807No Hit
CTGGGAGGTGCAGAACAGGACAGACCAGCGGGGTGGCATAGAGAAAGTCC11020.12041189171234014No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA10900.1191006914396105No Hit
CTGGGATTACCATTGCTCTGGGACCATCTTCTGAGTCAGACTTGGGGAGC10760.11757095778809254No Hit
CTGCTGCTTACCATAAACTATGAAAAGCACAGGTTTTAAGCCCGCCAGGA10720.11713389103051601No Hit
CTGCCCTCCTGTTTCGATTACGTGATGCACTTTCTGACTGTGTTCTGGAA10600.11582269075778634No Hit
CTTCTTCTTCTTGGCCCAGATATTGTGATAGTTCTCAGCCACGACCTCCA10380.1134188235911153No Hit
CTGTTTTCAGCCTGGTTCATTTGGTACAAGTGACTCAGAAAATGGCCTTT10290.11243542338656806No Hit
CTGGGGGCAGCATTTCTGGAATCCTCCTAACCATTGTTCACTGCTTGTAG10250.1119983566289915No Hit
CTGTTCTTACCTACAATGGAGAGAATCACCACCACAAAGTCAAAGATGTT10190.11134275649262668No Hit
AGATGCTGCTCAAATGGGTGGCCTACGGCTTTAAGGTGTACTTCACCAAC10140.110796423045656No Hit
CTTAGGCCCGGTTCATCTCAGATGTGTTTTCTAGGCCTGGGGCTGAAAGG10130.11068715635626186No Hit
CTGTGCCTCCGAACTGGGCTTTCTTCTCTTCTCCCTCACCATGGCCATCA10100.11035935628807944No Hit
CTGCTCAGCCCGAAGCAGGGCTCACACTTTTGAAGGTGGTTTCTCCTCTG10050.10981302284110875No Hit
CTGATTGCCCCTGGGCCCTAGTGAGAACACTCCAGCCTGGATATGTTCAG9960.10882962263656151No Hit
CTCCAAGGACCGCAGACTTCTGCTGAAGTGTACCGCATCCTCAAACAGAA9950.10872035594716738No Hit
CTGATCTCCAAGGTCCTTCCTAAGAATCTGGGATCTGGAGGATCCCAGGG9870.10784622243201426No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT9800.10708135560625531No Hit
CTTGCCATGTACAAGGGGGACCTGTTTCAGATATTCCATGGAGACCCTGG9770.10675355553807289No Hit
CTCTGAGCATCTTCCCCACCATCATCCTCCAAGTCATCAAGATCAGATTC9740.10642575546989047No Hit
CTCTGAGAGGCAGCAGGACTTGATCCAAACCCACAGTCTTGTCCTGGAGG9660.10555162195473736No Hit
CTGGTTAGTCGGAAGGCGTTTGTGGCTAAGGCCTTGAAAGGGAAAAGTCT9660.10555162195473736No Hit
CTCTCCCAGCAAGACACCTGAGGAAGTTAGCACCCCTGCAGAGGAGGAGA9650.10544235526534322No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA9650.10544235526534322No Hit
CTTGTTCTGCTTGTCTTCATGTCGCATCTCCTCCTCTGGGGGCTGGAAGT9580.10467748843958427No Hit
CTTGTACCCAGGAAGAGACATAGGCGAGAGGGGAGCAGTTGGTGAGGAGC9550.10434968837140185No Hit
CTCAAGACAGATGCCGGACAGGGCTGCTGCTCCGAAGGAAAAGGCTCCCA9400.10271068803048979No Hit
CTGTTTGGCGGCAAGTTTAATTTTGATGAAACGCAAACCAAGCGGAGCAC9350.10216435458351909No Hit
CTGCTACTTACCTCTGCTGCCAAGTAATGCCCCGTGGCCAGATGCTTGAA9270.10129022106836598No Hit
CTCCCAACTATTTGCCGGGAAGACCCAGAGATACATGGCTATTTCAGGGA9230.10085315431078944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)