FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403895
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATCTCGTA883346.292066002087051TruSeq Adapter, Index 9 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATATCGTA29610.2109132093212099TruSeq Adapter, Index 9 (97% over 39bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT29090.20720922861040178No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG27550.1962397472745469No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA15860.11297141167964841No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA14560.10371145990262803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)