FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351817
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTA853866.316387499195527TruSeq Adapter, Index 1 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG43090.318756163001353No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA31880.2358307374444914No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT27480.20328195310459923No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA25080.1855280707373853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)