FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713127
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTA25467614.866148277389826TruSeq Adapter, Index 20 (97% over 38bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG139120.8120822332494906No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA102170.5963947798382724No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT90900.5306086472281389No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA70630.41228700499145715No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT50760.296300274293733No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT40990.23927005995469106No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT36200.2113094942756725No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATATCGTA30540.17827049599942094TruSeq Adapter, Index 20 (97% over 38bp)
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA20480.11954747079463461No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAACCTCGTA20130.11750442319804662TruSeq Adapter, Index 20 (97% over 38bp)
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA17150.10010933223281168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)