FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517821
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTA21463314.140863777744542TruSeq Adapter, Index 2 (97% over 38bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG106910.7043650074679425No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT58230.3836420763713244No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA54770.36084623944457217No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT37910.24976594736797028No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATATCGTA37570.24752589402834724TruSeq Adapter, Index 2 (97% over 38bp)
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA33050.21774636139571135No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT24570.1618767957486423No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCACCTCGTA20170.13288787017704987TruSeq Adapter, Index 2 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGACCATCTCGTA18940.12478414780135472TruSeq Adapter, Index 2 (97% over 38bp)
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA18340.12083111249613755No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT16530.10890612265873248No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT15770.10389894460545743No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATTTCGTA15590.10271303401389228TruSeq Adapter, Index 2 (97% over 38bp)
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA15470.10192242695284885No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)