FastQCFastQC Report
Tue 7 May 2024
EGAF00001816566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001816566
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22987
Total Bases5.8 Mbp
Sequences flagged as poor quality0
Sequence length234-273
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA928340.38369513203115No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA434118.8845869404446No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA336314.630008265541395No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA14366.247009179101231No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA5052.196893896550224No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA3451.5008483055640145No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2250.9788141123243572No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA1750.7612998651411668No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGTGCA870.37847479009875146No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA740.32192108583112194No Hit
TACGTAGGTCCCGAGCGTTGTCCGGCTTTATTGGGCGTAAAGCGAGCGCA650.28276852133814767No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA380.16531082785922477No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA380.16531082785922477No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCACGCA360.15661025797189715No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAACGCGAGCGCA310.1348588332535781No Hit
TACGTAGGTGGCAAGCGTTGTCCGGCTTTATTGGGCGTAAAGCGAGTGCA300.1305085483099143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA260.11310740853525905No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGGGCGCA250.10875712359159524No Hit
TACGTCGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA240.10440683864793145No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAGGCGAGCGCA240.10440683864793145No Hit
TACGTAGGTCCCGAGCGTTGCCCGGATTTATTGGGCGTAAAGCGAGCGCA240.10440683864793145No Hit
TACGTAGGTGGCAAGCGTTATCCGGCTTTATTGGGCGTAAAGCGAGCGCA240.10440683864793145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)