FastQCFastQC Report
Tue 7 May 2024
EGAF00001816578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001816578
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences31221
Total Bases7.8 Mbp
Sequences flagged as poor quality0
Sequence length226-274
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA1112735.639473431344285No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA784425.124115178886008No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA21056.742256814323692No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA9963.1901604689151535No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA7192.3029371256526057No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA6722.152397424810224No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3661.1722878831555685No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA3471.1114314083469459No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA2420.7751193107203485No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGTA2360.7559014765702572No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1580.5060696326190705No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1280.4099804618686141No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1220.39076262771852277No Hit
TACGTAGGTGGCGAGCGTTGTCCGGCTTTACTGGGCGTAAAGGGAGCGTA1140.3651388488517344No Hit
TACGTAGGGGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA1040.33310912526824893No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA1030.3299061529099004No Hit
TACGTAGGGGGCTAGCGTTATCCGGCTTTACTGGGCGTAAAGGGTGCGTA980.31389129111815767No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA970.3106883187598091No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA770.24662887159283814No Hit
TACGTAGGTGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA770.24662887159283814No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGCA620.19858428621760993No Hit
TACGTAGGAGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA600.19217834150091284No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA500.16014861791742738No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA470.15053970084238172No Hit
TACGTAGTGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA460.14733672848403317No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA460.14733672848403317No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTACAGGGAGCGTA450.14413375612568463No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGCGCGTA440.1409307837673361No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAGGGGAGCGTA430.13772781140898754No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA420.134524839050639No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGCA380.12171294961724481No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTCAAGGGAGCGTA370.11850997725889627No Hit
TACGGAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA370.11850997725889627No Hit
CACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA370.11850997725889627No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA360.11530700490054771No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGCGTA330.10569808782550207No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAACGGGAGCGTA330.10569808782550207No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGTA330.10569808782550207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)