FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001816761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001816761
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19452
Sequences flagged as poor quality0
Sequence length226-254
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA836743.013571869216534No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA13216.791075467818219No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA11956.143327164301871No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA6243.2078963602714374No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5562.858317910754678No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA4802.4676125848241828No Hit
TACGAAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTGTGTA4322.2208513263417644No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA3992.051202961135102No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTA2781.429158955377339No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA2101.0795805058605799No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1840.9459181575159367No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1380.7094386181369525No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA1240.6374665844129138No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGCA1030.5295085338268559No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA970.49866337651655357No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA940.48324079786140245No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAGGGAGCGTA870.4472547809993831No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA700.35986016862019327No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA690.35471930906847626No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA640.32901501130989097No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA580.29816985399958873No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA560.28788813489615467No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA510.26218383713756943No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGAGAGCGTA500.2570429775858524No Hit
TACGGAGGATCCGAGCGTTATCCGGCTTTATTGGGTTTAAAGGGAGCGTA490.2519021180341353No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGAGTGCGTA480.24676125848241826No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGCA460.23647953937898417No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA450.23133867982726714No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA450.23133867982726714No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA430.22105696072383302No Hit
TACGGGGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA430.22105696072383302No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA430.22105696072383302No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA430.22105696072383302No Hit
TACGTAGGGAGCGAGCGTTATCCGGAATTATTGGGTGTAAAGGGTGCGTA400.20563438206868187No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA380.19535266296524778No Hit
TACGGAGGGTCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA370.19021180341353075No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA370.19021180341353075No Hit
TACGGAGGATCCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGAGCGTA370.19021180341353075No Hit
TACGGAGGATCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA360.1850709438618137No Hit
CACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA320.16450750565494549No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA300.15422578655151142No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGAGAGCGCA300.15422578655151142No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGCGCGTA290.14908492699979436No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGAGTA290.14908492699979436No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA290.14908492699979436No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGCGAGCGTA280.14394406744807733No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTG270.13880320789636028No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA270.13880320789636028No Hit
TACGTAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA270.13880320789636028No Hit
TACGGGGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA260.13366234834464322No Hit
TACGGAGGATCCGAGCGTTATCCGGGTTTATTGGGTTTAAAGGGAGCGTA260.13366234834464322No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGCA250.1285214887929262No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGTGAGCGTA230.11823976968949208No Hit
TACGGAGGATCCGAGCGTTATCCGGACTTATTGGGTTTAAAGGGAGCGTA230.11823976968949208No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGAGAGCGCA230.11823976968949208No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGCGAGCGTA230.11823976968949208No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA230.11823976968949208No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGTGCGTA220.11309891013777504No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA210.107958050586058No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTA210.107958050586058No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA200.10281719103434094No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA200.10281719103434094No Hit
TACGGAGGCTCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA200.10281719103434094No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGTA200.10281719103434094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)