FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001816909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001816909
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences30959
Sequences flagged as poor quality0
Sequence length227-264
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA1607551.92351174133532No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA29219.435059271940307No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA11343.662909008688911No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA8022.590522949707678No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA7772.5097709874349947No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA6362.0543299202170613No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4731.5278271261991665No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA4601.4858361058173712No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA4491.4503052424173908No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA4161.3437126522174487No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA2890.9334926838722181No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA2060.6653961691269097No Hit
TACAGAGGTCTCAAGCGTTGTCCGGAATCACTGGGCGTAAAGCGTGCGTA1320.4263703607997675No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1320.4263703607997675No Hit
TACGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA910.293937142672567No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA910.293937142672567No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA830.2680965147453083No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA760.245485965308957No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC760.245485965308957No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA760.245485965308957No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA720.2325656513453277No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAGGCGTGCGTA680.21964533738169836No Hit
TACGGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA660.21318518039988374No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAACCACTGGGCGTAAAGCGTGCGTA650.2099551019089764No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAGTCACTGGGCGTAAAGCGTGCGTA640.20672502341806906No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA600.19380470945443976No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTGCGTA580.18734455247262508No Hit
TACAGAGGTCTCAAGCGTTGTTCGGGATCACTGGGCGTAAAGCGTGCGTA560.18088439549081042No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGCA560.18088439549081042No Hit
TACAGGGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA550.17765431699990308No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAGAGCGTGCGTA540.17442423850899577No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA530.17119416001808843No Hit
TACAGAGGTCTCAAGCGTTGTTCGGCATCACTGGGCGTAAAGCGTGCGTA510.16473400303627378No Hit
TACAGAGGTCTCGAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA500.16150392454536644No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA500.16150392454536644No Hit
TACAGAGGACTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA490.15827384605445913No Hit
TACAGAGGTCTCAGGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA460.14858361058173714No Hit
CACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA440.14212345359992248No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA440.14212345359992248No Hit
TACAGAGGTCCCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA440.14212345359992248No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA430.13889337510901514No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTC410.1324332181272005No Hit
GTTTTAATTTTTGTAGAGAAGGAGTCTTGCTATGTTGCCCAGGCTGGTCT390.12597306114538584No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCGCGTA390.12597306114538584No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA360.11628282567266385No Hit
TACAGAGGTCTCAAGCGTTGCTCGGAATCACTGGGCGTAAAGCGTGCGTA350.11305274718175651No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA350.11305274718175651No Hit
TACGTAGGTGACAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA340.10982266869084918No Hit
TACAGAGGCCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA340.10982266869084918No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA340.10982266869084918No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTG340.10982266869084918No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA330.10659259019994187No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTGAAGCGTGCGTA330.10659259019994187No Hit
TACGTAGGGGACAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA330.10659259019994187No Hit
TACAGAGGTCTCAAGCGTTGTTCAGAATCACTGGGCGTAAAGCGTGCGTA310.10013243321812719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)