FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001816919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001816919
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences12153
Sequences flagged as poor quality0
Sequence length234-257
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA446136.70698592940015No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA166413.692092487451657No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA9507.8169999177157905No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4823.9660989056200115No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3983.274911544474615No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2612.147617872130338No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1741.431745248086892No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1681.3823747222907923No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1641.3494610384267258No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTA1341.1026084094462274No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA1120.9215831481938617No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA1120.9215831481938617No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA960.7899284127375956No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA760.6253599934172632No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA690.5677610466551469No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA620.5101620998930305No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGAGAGCGTA490.4031926273348144No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA470.3867357854027812No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA440.3620505225047313No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA420.34559368057269807No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAGGGAGCGTA390.3209084176746482No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA340.27976631284456516No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA330.27153789187854854No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGAGAGTGTA310.25508104994651526No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGTGTA300.24685262898049865No Hit
TACGGAGGATCCGAGCGTTATCCGGCTTTATTGGGTTTAAAGGGAGCGTA290.23862420801448203No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA260.21393894511643213No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA250.20571052415041552No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA230.1892536822183823No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA230.1892536822183823No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGAGGGAGCA230.1892536822183823No Hit
TACGGAGGGTCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA210.17279684028634903No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA200.16456841932033242No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA200.16456841932033242No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTG200.16456841932033242No Hit
TACGTAAGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGTGCGTA200.16456841932033242No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA190.1563399983543158No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA190.1563399983543158No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGCTTAAAGGGAGTGTA180.1481115773882992No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGAGTGCGTA180.1481115773882992No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA180.1481115773882992No Hit
TACGGCGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA170.13988315642228258No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA170.13988315642228258No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGCA160.13165473545626594No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA160.13165473545626594No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAGGGGAGCGTA160.13165473545626594No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA160.13165473545626594No Hit
TACGGACGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA160.13165473545626594No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTC150.12342631449024932No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGGTTAAAGGGAGCGTA150.12342631449024932No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTGAAGGGAGCGTA140.11519789352423271No Hit
TACGGGGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA140.11519789352423271No Hit
TACGGAGGATCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA140.11519789352423271No Hit
TACGGGGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA140.11519789352423271No Hit
TACGGAGGCTCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA130.10696947255821607No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA130.10696947255821607No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGTA130.10696947255821607No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTCTAAAGGGAGCGTA130.10696947255821607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)