FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001816944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001816944
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21261
Sequences flagged as poor quality0
Sequence length230-257
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1251858.87775739617139No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA9364.402426978975589No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA5422.549268613893984No Hit
TACGGAGGGTACAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA5012.356427261182447No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4442.0883307464371383No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4201.9754480033864823No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3981.8719721555900477No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA3341.5709515074549645No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2481.1664550115234467No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2281.0723860589812333No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA1590.7478481727105969No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1570.7384412774563756No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA1340.6302619820328301No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1290.6067447438972767No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA1150.5408964771177273No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA1110.5220826866092846No Hit
AACTAACGATCGCCTACTGGGGAGCAGAGTTTCTACCCAGAAGAGGAAAC890.4186068388128498No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA780.3668689149146324No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA710.33394478152485774No Hit
TACGGAGGGTGCAAGCGTTAACCGGAATTACTGGGCGTAAAGCGCACGCA640.30102064813508306No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA640.30102064813508306No Hit
TACGGAGGGTGCAAGCGTTAATCGGGATTACTGGGCGTAAAGCGCACGCA620.29161375288086167No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA610.286910305253751No Hit
TACGGAGGGTGCAAGCGTTGATCGGAATTACTGGGCGTAAAGCGCACGCA570.2680965147453083No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAGGCGCACGCA530.24928272423686565No Hit
TACGGAGGGTGCAAGCGTTAATCGGCATTACTGGGCGTAAAGCGCACGCA520.24457927660975495No Hit
TACGGAGGGTGCAAGCGTTAATCGGAGTTACTGGGCGTAAAGCGCACGCA520.24457927660975495No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA510.23987582898264428No Hit
TACGGAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA460.21635859084709091No Hit
TACGGAGGGTGCAAGCGTTAGTCGGAATTACTGGGCGTAAAGCGCACGCA450.21165514321998025No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA430.2022482479657589No Hit
TACGGCGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA410.19284135271153754No Hit
TACGGAGGGTGCAAGCGCTAATCGGAATTACTGGGCGTAAAGCGCACGCA370.17402756220309487No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCG370.17402756220309487No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA340.15991721932176287No Hit
TACGTAGGTGACGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA320.15051032406754153No Hit
TACGGGGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA310.14580687644043083No Hit
AACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA310.14580687644043083No Hit
TACGGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCACGCA300.14110342881332016No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCC300.14110342881332016No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA300.14110342881332016No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAGAGCGCACGCA300.14110342881332016No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA290.1363999811862095No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA270.12699308593198813No Hit
TACGGAGGGTGCAAGCGTTAATCAGAATTACTGGGCGTAAAGCGCACGCA270.12699308593198813No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGCA270.12699308593198813No Hit
TGCGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA250.1175861906777668No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGTGTAAAGCGCACGCA240.11288274305065614No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA240.11288274305065614No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGAGTA240.11288274305065614No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCACACGCA240.11288274305065614No Hit
TATGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA230.10817929542354546No Hit
CACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA230.10817929542354546No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTGCTGGGCGTAAAGCGCACGCA220.10347584779643478No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA220.10347584779643478No Hit
TACGTAGGGGACAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA220.10347584779643478No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA220.10347584779643478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)