FastQCFastQC Report
Tue 7 May 2024
EGAF00001816976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001816976
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences141164
Total Bases35.6 Mbp
Sequences flagged as poor quality0
Sequence length239-265
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGCA2641318.710861126066135No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1897713.443229151908417No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1564411.082145589527075No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGCA82935.874727267575302No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA77915.5191125216060755No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA71305.050862826216316No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA53573.7948768807911364No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA52743.7360800204018023No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA37202.6352327788954693No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA35082.4850528463347596No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA33352.3625003541979543No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA23231.645603694993058No Hit
TACGGAGGGTGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGAGTTCGTA14321.0144229406930945No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTA13070.9258734521549403No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA12480.8840780935649316No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA8430.597177750701312No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA6410.4540817772236547No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTCATTGGGCGTAAAGAGCGCGTA5840.41370321045025643No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA5810.4115780227253407No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA4100.2904423224051458No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA3910.27698280014734633No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTACGTA3620.25643931880649457No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA3560.25218894335666314No Hit
AACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA3490.24723017199852654No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGGGCATGCA2590.1834745402510555No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA2560.18134935252613982No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2520.17851576889291887No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA2510.17780737298461363No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGCA2450.17355699753478224No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA2190.15513870391884618No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGCA2130.15088832846901476No Hit
TACGGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA2120.15017993256070955No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2040.14451276529426765No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA1830.12963645121985776No Hit
TACGTAGGGGGCAAGCGTTAATCGGAATAACTGGGCGTAAAGGGCATGCA1800.12751126349494207No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1740.12326088804511066No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA1730.12255249213680541No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1690.11971890850358449No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTACGTA1640.11617692896205832No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1620.11476013714544786No Hit
TACGTAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1500.10625938624578504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)