FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001817095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001817095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873257
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCCCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCA139270.743464457893391No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCCTCAGGCTGCAGGCT88960.4748947955352629No Hit
TTATAGTACTCCCCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC83980.4483100823859193No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT61620.328945788004529No Hit
CTTTATTACAGATCCAATGATAAGTACTGCTTAGCTTGGTACCAGCAGAA60360.3222195352799963No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58790.313838410853396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG49430.26387196204258145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48540.25912087876890355No Hit
GATAAGTACTGCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAA48270.25767953889936085No Hit
GTACTATAATACTGCTGACAGTAATAAACTGCCACATCCTCAGGCTGCAG45040.24043684342297933No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40530.21636112930580267No Hit
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT39210.20931457883248267No Hit
TTATTACTGTCAGCAGTATTATAGTACTCCCCTCGCTTTCGGCGGAGGGA37680.2011469862384072No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34420.18374414188763208No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA31780.16965104094099206No Hit
TCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC31530.16831646698771177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30560.1631383200489842No Hit
ATAGTACTCCCCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA30030.16030902326802995No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG28660.1529955580040539No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.1485113895210321No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27490.1467497519027021No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27370.14610915640512753No Hit
GTACTGCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGC26920.143706923289223No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT25730.13735435127160875No Hit
GTAATAAACTGCCACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA25510.1361799261927221No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25080.13388445899307996No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGCAGTACTTA24950.13319048053737423No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24810.13244311912353723No Hit
GCTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATT24100.12865292909622117No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC23510.12550333456647966No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC23400.12491612202703634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.12459582427824907No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT23240.12406199469693693No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23100.12331463328309995No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22260.11883046480007815No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA21130.11279819053125119No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG21110.11269142461498877No Hit
TTCGGGGACATCGTGATGACCCAGTCTCCGGACTCCCTGGCTGTGTCTGT21100.11263804165685753No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG20410.10895461754580392No Hit
GTCTGTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTT20350.10863431979701663No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG20320.108474170922623No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19820.10580502301606239No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19590.10457721497904451No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19550.10436368314651967No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19410.1036163217326827No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19280.10292234327697694No Hit
ATTATAGTACTCCCCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA18990.1013742374911718No Hit
ATAATACTGCTGACAGTAATAAACTGCCACATCCTCAGGCTGCAGGCTGC18800.10035996128667876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)